Autoradiograms away from steroid metabolites off vitellogenic ovarian hair follicles of bambooleaf wrasse incubated which have [7- step 3 H]pregnenolone

Autoradiograms away from steroid metabolites off vitellogenic ovarian hair follicles of bambooleaf wrasse incubated which have [7- step 3 H]pregnenolone

7 metabolites was split by narrow layer chromatography and you may arranged that have benzene:acetone (4:step 1). step one, E1; dos, AD; step 3, P5; cuatro, DHEA 17-P; 5, E2; six, 17-P5; eight, 17,20?-P 17,21-P; and you may 8, 17,20?-P5. See Table step 1 to possess steroid abbreviations.

Dining table step 3

To determine the steroidogenic pathway out-of E2 synthesis, four almost every other radiolabeled steroid drugs were utilized once the precursors to own inside vitro incubation out-of hair follicles. Of them five precursors, E2 was synthesized of [ 14 C]DHEA, [ fourteen C]Advertising, and you may [ 3 H]E1. When hair follicles was in fact incubated which have [ 3 H]17-P, E2 was not imagined since metabolite, and you can a small amount of Ad are produced. In all incubations, T creation couldn’t getting perceived, and you can [ fourteen C]T wasn’t completely transformed into E2.

Dimensions regarding serum steroid membership

Solution quantities of E2 and E1 presented similar pages within the a great day’s spawning year (Fig. 2). On hour, if developmental phase of the prominent oocytes are LMN, serum E2 and you may E1 account was indeed rather higher (E2= ng/ml, E1= 5.08 ng/ml). But by hour, when the stage of largest oocytes try M, the levels fell (E2=5.03 ng/ml, Estep one=step 1.fifty ng/ml) and you may stayed ongoing levels during the and hr, if the levels of premier oocytes was TY and you can EMN, correspondingly (E2=6.00 ?six.twenty-seven ng/ml, E1=1.step one5–step 1.79 ng/ml). not, T and Advertising membership was basically lingering (T=step 1.22–step one.71 ng/ml, AD=1.23–step one.43 ng/ml) all round the day and you can did not disagree notably one of ovarian levels (Fig. step three).

Fig. 2

Changes in the serum levels of estradiol-17? (E2) and estrone (E1) in female bambooleaf wrasse sampled at different times of a day during the spawning season. Numbers indicate sample size. Each value is the mean±SEM. Statistically significant differences between groups are shown by different letters (P<0.05).

Fig. 3

Changes in gel degrees of testosterone (T) and you may androstenedione (AD) inside girls bambooleaf wrasse sampled on differing times out of day inside spawning 12 months. Wide variety imply take to proportions. Each well worth ‘s the indicate±SEM.


This study shows the new steroidogenic pathway so you can E2 synthesis in the ovarian hair follicles off bambooleaf wrasse (Fig. 4, vitellogenic path). During the last twenty years, a lot of knowledge into steroidogenesis during the vitellogenesis, in various teleosts, provides incubated undamaged ovarian hair follicles otherwise fragments otherwise mentioned bloodstream steroid accounts. in the vitro incubation off ovarian hair follicles (amago salmon Oncorhynchus rhodurus , Kagawa ainsi que al., 1982; goldfish, Kagawa mais aussi al., 1984; guppy Poecilia reticulata , Venkatesh ainsi que al., 1992; medaka Oryzias latipes, Kobayashi mais aussi al., 1996) otherwise ovarian fragments (age.grams., Atlantic croaker Micropogonias undulatus , Trant and you may Thomas, 1989) displayed one to E2 was synthesized through T. In numerous varieties, it’s been found that bloodstream E2 account boost while in the vitellogenesis, in addition to T profile (elizabeth.g., plaice Pleuronectes platessa , Wingfield and you can Grimm, 1977; white sucker Catostomus commersori , Scott ainsi que al., 1984; noticed seatrout Cynoscion nebulosus , Thomas et al., 1987; red seabream Pagrus significant, Matsuyama ainsi que al., 1988; gulf of mexico killifish Fundulus grandis , Greeley ainsi que al., 1988; ocean bass Dicentrarchus labrax , Prat ainsi que al., 1990; demoiselles Chromis dispilus , Barnett and Pankhurst, 1994; striper Morone saxatilis , King mais aussi al., 1994; spiny damselfish Acanthochromis polyacanthus , Pankhurst mais aussi al., 1999). These studies possess led us to this new acknowledged evaluate you to definitely T try a precursor to possess E2 manufacturing regarding teleost ovary. Inside our investigation, yet not, T wasn’t developed by vitellogenic follicles immediately after incubation having radiolabeled precursors, and you will exogenous T wasn’t transformed into E2 regarding follicles. This type of performance obviously indicate that inside the bambooleaf wrasse T is not the brand new predecessor getting E2 creation on vitellogenic follicles. In contrast, E1 was produced in place of T off P5, DHEA, and you can Advertising, and you will exogenous E1 is actually translated into E2. The outcomes suggest that E2 is synthesized away from E1 from the vitellogenic follicles. Moreover, the fresh solution steroid accounts, where solution E2 levels exhibited good diurnal rhythm that paralleled E1 membership yet not T account, hold the into the vitro data. To your knowledge, here is the very first report that E1 ‘s the biggest sandwich-stratum out of E2 throughout the ovarian follicles in an excellent teleost.